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The genetic basis of Czech garlic "paličák" (A. sativum L. ssp. ophioscorodon) focusing on the biosynthetic pathways of the secondary metabolites
Čermák, Vladimír ; Ovesná, Jaroslava (advisor) ; Demnerová, Kateřina (referee)
Czech bolting garlic (Allium sativum L.) is the name for Czech varieties, which are categorized into subspecies sativum ssp. An inflorescence production and specific onion morphology is typical for this type of garlic. The genetic basis, that has been described by the analysis of microsatellites, are discussed in this thesis. Transcription analysis has revealed a large number of unigenes that have been assigned by genetic ontology to individual functions in the organism. Therefore, genetic differences from other varieties were confirmed. The impact of the environment and cultivation practices, including large- scale production, is being explored. The results can be used for further research or breeding. Consumers and experts have rated the Czech bolting garlic as a variety with a distinctly pungent taste and aroma. Substances causing this strong characteristic taste are secondary metabolites, alk(en)ylcysteine-S-oxides (ACSO), especially alliin and methiin. Chemical analysis did not confirm the exceptionally high value of these taste precursors or other substances that should distinguish Czech bolting garlic. Secondary metabolism includes other important substances that are used mainly in pharmacy and healthcare, where garlic has been used for thousands of years due to antibacterial effects,...

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